Cell-particle association rate calculator

Static Adherent
Well-mixed Adherent
Suspension
About

Description

Static adherent incubation is the most common type of nanomaterial incubation experiment. In it, adherent cells are placed on the bottom of plate wells, and a well mixed solution of nanoparticles is introduced. Over time these particles settle to the bottom and are taken up by cells. This methodology requires more extensive chracterization and details of the experiment than the others here, but is the easiest and most commonly performed in vitro experiment. We assume spherical particles and a cylindrical plate well.

Experimental details

Enter the details of your experiment and characterization here. If you're not sure about a particular field, hover over the for more information.

Description

Well-mixed adherent cell incubation is a less common experimental condition that requires you to continuously mix the solution that your adherent cells are incubated in. It has few characterization requirements, but needs special devices to continuously mix. It can be used to compare non-spherical nanocarriers. We assume the plate well is cylindrical.

Experimental details

Enter the details of your experiment and characterization here. If you're not sure about a particular field, hover over the for more information.

Description

Suspension incubation is another common experimental condition, using suspension cells. We assume that suspension cells and nanocarriers remain well-mixed over the duration of the experiment, and that means that suspension incubation also has few characterization requiement, and can be used to comparen non-sphereical particles.

Experimental details

Enter the details of your experiment and characterization here. If you're not sure about a particular field, hover over the for more information.

This is a companion site to the paper Revisiting cell–particle association in vitro: A quantitative method to compare particle performance

You can use this site to calculate the association rate of your nano or bio-engineered materials, whether they be inorganic, organic, or something in between, using the exact same method used in linked manuscript. Our hope is that this will facilitate quantiative comparison of different bio-nanomaterials at the pre-clinical stage. Please cite this paper if you use this website on your results.

If you're interested in collaborating further, please reach out to the first and/or corresponding authors! This website uses the "Cell Carrying Capacity" or CCC model, as it was found to have the best performance of all candidate models.